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Health Encylopedia

 
Buccal smear
 
SubjectContents
Definition A test where cells are taken from the tongue and evaluated for the presence of Barr bodies (a mass seen in a normal female sex chromosome).
Alternative Names Sex chromatin test
How the test is performed Cells are collected by scraping the tongue with a spatula. The cells are then placed on a slide and the sample is taken to the laboratory for evaluation.
How to prepare for the test No preparation is necessary for this test.
How the test will feel You will feel a scraping sensation as cells are removed from the tongue.
Why the test is performed In the past, this test was used to indicate:
  • abnormal sexual development
  • ambiguous genitalia
  • amenorrhea
  • suspected chromosomal abnormalities
  • Today, a health care provider who suspects any of these abnormalities is most likely to perform a full chromosome analysis (called karyotyping ). The buccal smear is primarily used in the Olympics or other sporting event if the authorities believe a man is trying to compete as a woman.
    Normal Values The normal value depends on the reason for the screen. If a male if suspected of imitating a woman, then a normal result would indicate the presence of Barr bodies, or that the person really is a female.
    What abnormal results mean If no Barr bodies appear the test subject is a male.
    What the risks are There are no risks associated with the buccal smear.
    Special considerations 
      

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